| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
24/02/2015 |
|
Data da última atualização: |
23/05/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BRAGA, R. M.; SANTANA, M. F.; COSTA, R. V. da; BROMMONSCHENKEL, S. H.; ARAÚJO, E. F. de; QUEIROZ, M. V. de. |
|
Afiliação: |
RODRIGO VERAS DA COSTA, CNPMS. |
|
Título: |
Transposable elements belonging to the Tc1-Mariner superfamily are heavily mutated in Colletotrichum graminicola. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
Mycologia, New York, v. 106, n. 4, p. 629-641, 2014. |
|
DOI: |
10.3852/13?262 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Transposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the characterized elements are autonomous in the sequenced isolate. The possible existence of active copies in field isolates from Brazil was shown. The TCg1 element is present in severalC. graminicola isolates and is a potentially useful molecular marker for population studies of this phytopathogen. Key words: RIP, transposase, transposon MenosTransposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the char... Mostrar Tudo |
|
Thesagro: |
Antracnose. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02476naa a2200205 a 4500
001 2009650
005 2017-05-23
008 2014 bl uuuu u00u1 u #d
024 7 $a10.3852/13?262$2DOI
100 1 $aBRAGA, R. M.
245 $aTransposable elements belonging to the Tc1-Mariner superfamily are heavily mutated in Colletotrichum graminicola.$h[electronic resource]
260 $c2014
520 $aTransposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the characterized elements are autonomous in the sequenced isolate. The possible existence of active copies in field isolates from Brazil was shown. The TCg1 element is present in severalC. graminicola isolates and is a potentially useful molecular marker for population studies of this phytopathogen. Key words: RIP, transposase, transposon
650 $aAntracnose
700 1 $aSANTANA, M. F.
700 1 $aCOSTA, R. V. da
700 1 $aBROMMONSCHENKEL, S. H.
700 1 $aARAÚJO, E. F. de
700 1 $aQUEIROZ, M. V. de
773 $tMycologia, New York$gv. 106, n. 4, p. 629-641, 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
06/03/2020 |
|
Data da última atualização: |
15/07/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
NOMURA, E. S.; CUQUEL, F. L.; DAMATTO JUNIOR, E. R.; BEZERRA, D. P.; BORGES, A. L. |
|
Afiliação: |
EDSON SHIGUEAKI NOMURA, APTA; FRANCINE LORENA CUQUEL, UFPR; ERVAL RAFAEL DAMATTO JUNIOR, APTA; DANIELA PEIXOTO BEZERRA, USP; ANA LUCIA BORGES, CNPMF. |
|
Título: |
Post-harvest characterization of 'Prata' banana cultivar grown under different nitrogen and potassium fertilization. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Revista Brasileira de Fruticultura, v.41, n.4, Jaboticabal, Ago. 2019. |
|
ISSN: |
1806-9967 |
|
Idioma: |
Inglês |
|
Conteúdo: |
There are several banana cultivars resistant to Black Sigatoka disease and to Panama disease, but due to the lack of information regarding post-harvest characteristics, many are rejected in the market. Nitrogen and potassium fertilization may help to improve the sensory characteristics of ripe banana, but there is lack of studies on its effects on banana cultivation, especially those resistant to diseases. The aim of this study was to evaluate the post-harvest characteristics of ?Prata-Anã?, ?BRS Platina? and ?PA94-01? banana cultivars at four N and K2O fertilization levels (NK0: no fertilization, NK1: 175 and 285 kg ha-1 year-1, NK2: 350 and 570 kg ha-1 year-1, NK3: 525 and 855 kg ha-1 year-1, respectively) in three production cycles. It could be concluded that N and K doses adopted in this work did not influence the post-harvest physicochemical characteristics of ?Prata-Anã?, ?BRS Platina? and ?PA94-01? banana cultivars. Regardless of N and K doses, ?Prata-Anã?, ?BRS Platina? and PA94-01? banana cultivars presented differences regarding post-harvest physicochemical characteristics. Production cycles did not influence the physical characteristics but differed in post-harvest chemical characteristics in ?Prata-Anã?, ?BRS Platina? and ?PA94-01? banana cultivars. |
|
Thesagro: |
Banana. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/211594/1/Post-harvest-characterization.pdf
|
|
Marc: |
LEADER 01896naa a2200193 a 4500
001 2121014
005 2020-07-15
008 2019 bl uuuu u00u1 u #d
022 $a1806-9967
100 1 $aNOMURA, E. S.
245 $aPost-harvest characterization of 'Prata' banana cultivar grown under different nitrogen and potassium fertilization.$h[electronic resource]
260 $c2019
520 $aThere are several banana cultivars resistant to Black Sigatoka disease and to Panama disease, but due to the lack of information regarding post-harvest characteristics, many are rejected in the market. Nitrogen and potassium fertilization may help to improve the sensory characteristics of ripe banana, but there is lack of studies on its effects on banana cultivation, especially those resistant to diseases. The aim of this study was to evaluate the post-harvest characteristics of ?Prata-Anã?, ?BRS Platina? and ?PA94-01? banana cultivars at four N and K2O fertilization levels (NK0: no fertilization, NK1: 175 and 285 kg ha-1 year-1, NK2: 350 and 570 kg ha-1 year-1, NK3: 525 and 855 kg ha-1 year-1, respectively) in three production cycles. It could be concluded that N and K doses adopted in this work did not influence the post-harvest physicochemical characteristics of ?Prata-Anã?, ?BRS Platina? and ?PA94-01? banana cultivars. Regardless of N and K doses, ?Prata-Anã?, ?BRS Platina? and PA94-01? banana cultivars presented differences regarding post-harvest physicochemical characteristics. Production cycles did not influence the physical characteristics but differed in post-harvest chemical characteristics in ?Prata-Anã?, ?BRS Platina? and ?PA94-01? banana cultivars.
650 $aBanana
700 1 $aCUQUEL, F. L.
700 1 $aDAMATTO JUNIOR, E. R.
700 1 $aBEZERRA, D. P.
700 1 $aBORGES, A. L.
773 $tRevista Brasileira de Fruticultura$gv.41, n.4, Jaboticabal, Ago. 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
